Biofilm & Lyme Disease

Biofilms: The Genesis of Antibiotic-Resistant Bacteria in Lyme Disease

What is Biofilm?

Biofilm is a protective shield manufactured by invading organisms to escape attacks from our antibodies and natural killer cells (immune system). Biofilm consists of a polysaccharide extracellular matrix described by microbiologists as a “super glue-like” substance.

Lyme spirochetes, Bartonella, and the mosquito parasite, Protomyxzoa Rheumatica, will wrap themselves in Biofilm if they are not immediately destroyed upon entry into our bloodstream. Thinking in three-dimensional terms, visualize Biofilm “bubbles” floating among red blood cells throughout the bloodstream as seen below and blood smears from Fry Laboratory.

Biofilm buster for biofilm eradication protocol

In 2015, Dr. Sponaugle made great advances in the clinic’s neurological protocol for “waking up” the neurotoxic brain of Lyme Disease patients. This has enabled the majority of his patients to begin destroying Biofilm within two to three weeks after starting treatment*.

Why Do We Care About Biofilm?

Clinical research at Sponaugle Wellness Institute has proven the quantity and consistency of Biofilm determine a multitude of outcomes.

We believe two things cause chronic Lyme disease:

  1. Suppressed Immune System
  2. Biofilm Formation

Biofilm would not exist if the patient were not already immunocompromised. This is why antibiotics do not work if you do not address the biofilm first.

Biofilm causes the “waxing and waning” – or feeling better temporarily after receiving antibiotic therapy – that many chronic Lyme disease patients experience during the rollercoaster of long-term IV antibiotics.

These patients feel better temporarily after antibiotics effectively kill spirochetes in the free-floating bloodstream, where many Lyme disease symptoms originate. The problem is that antibiotic therapy does not kill the spirochetes located inside the Biofilm formations that are floating through the bloodstream.

Several months later, the Biofilm-ridden spirochetes have produced enough offspring to reach a new critical mass effect, and they begin busting through the Biofilm.

These spirochetes travel through the free-floating bloodstream, where they attack joints and multiple other organs, most concerning of which is the brain. For this reason, it is important to eradicate Biofilm formations in Lyme disease patients.

Biofilm test results

Should Antibiotics Be Used For Tickborne Disease ?

As my research at Sponaugle Wellness has shown, there is a place for the use of IV antibiotics in chronic Lyme patients once the body's immune function and natural killer power has been optimized.

When supplementing a healthy immune system, antibiotic therapy will provide a more “desired kill” of Borrelia spirochetes, and get patients home faster. It should be noted that withholding antibiotic therapy in Neurological Lyme Disease patients demonstrates a lack of judgment.


Montoya, stacia biofilm
Swartz, terry biofilm

Biofilm Causes Antibiotic-Resistant Bacteria

While antibiotics can be very effective for killing Lyme Disease spirochetes and other bacteria in the “free-floating” bloodstream, antibiotics do not effectively penetrate the four-layer outer wall of Biofilm formations.

However, a small number of antibiotics can penetrate the Biofilm, but just enough to cause antibiotic resistance in Biofilm-protected bacteria.

Recent studies from the Center for Biofilm Engineering at Montana State University (MSU) validate this concept. The center’s research has proven that antibiotics do not effectively penetrate the protective Biofilm produced by Lyme disease spirochetes and other tick-borne microorganisms.

Microbiologists at MSU have stated, “Antibiotic therapy not only fails to produce a bactericidal-kill in Biofilm-protected organisms, but antibiotic therapy given before Biofilm has been adequately dissolved also induces bacterial mutations, creating even more resistant phenotypes.”

According to the American Association of Quantum Medicine (AAQM), the more we use antibiotics to treat Biofilm-producing microorganisms, the stronger and more resistant they become. Furthermore, AAQM suggests that Biofilm-producing, drug-resistant micro-organisms can only be conquered by enhancing the killing power of the immune system.

For this reason, the Sponaugle Wellness Team has spent years designing and improving Integrative Lyme Disease Treatment, which enhances immune function, even in the most immunocompromised Lyme disease patients.

As a result, our patients have no difficulty destroying Biofilm.

In addition to destroying Biofilm, we also optimize brain function, which optimizes the immune function, as the brain ultimately modulates and activates an immune function.

Case Study: Excess Antibiotics for Chronic Lyme Disease

Carol received lyme disease treatment at the sponaugle wellness institute in oldsmar, fl.
Carol - Before Treatment
Carol from new jersey lyme disease treatment testimonial
Carol - After Treatment

Patient: Carol from New Jersey

Background: Prior to finding Sponaugle Wellness Institute, Carol was treated for six years by the best and brightest LLMDs in New Jersey and New York. Over that six-year period, she was prescribed multiple antibiotics. Most significant is the fact that she was prescribed four grams a day of IV Rocephin for a total of three years. Unfortunately, the Rocephin was ineffective in killing the massive Bartonella infection seen within biofilm formation* on her blood smear.

Diagnoses: Lyme Disease, Bartonella, Mold Toxicity, Industrial Toxins

Symptoms: Chronic Fatigue (Bedridden), Brain Fog, Anxiety, Insomnia, Depression, Malnutrition

Teaching Point: Carol was the first patient in which Dr. Sponaugle was able to determine that Mold Toxins and Industrial Toxins are sequestered in Biofilm formations. Excessive antibiotics are not effective for Lyme Disease patients who have biofilm saturated with lipophilic Mold and Industrial Toxins that suppress the immune system and natural kill power of the body to fight off infection and disease.

Biofilm eradication protocol for lyme disease
Biofilm Test for Carol
Biofilm buster treatment testimonial

The last three years of excessive antibiotic treatment rendered Carol essentially bedridden.

Carol’s story is a good case study for the concept that antibiotics are ineffective, if not worthless, in Lyme patients who have developed significant biofilm. Carol and her husband are one of the nicest couples I have met through 30 years of practicing medicine.

“Carol’s Lyme Disease Treatment made her worse than the Lyme Disease infection itself, much like my daughter, Alexa, who had the same experience in her treatment,” said Dr. Sponaugle. “Our Lyme disease treatment in 2012 was not nearly as advanced as it is in 2016, and I credit Carol’s amazing transformation – in just three months* – to the fact that she is truly a Christian woman who sincerely prayed for, and believed in her recovery.”

“Because Carol’s husband was a brilliant mathematician, he wanted, like me, to prove or disprove concepts with numerical data, not guesswork,” said Dr. Sponaugle. “Therefore he allowed me to perform testing precisely before and immediately after we used our “biofilm-busting” IV Infusions.”

“To prove many of my theories through clinical research, we have had to eliminate variables, perform biomarker testing with precise before-and-after time frames, and use deductive reasoning. Patients remain the best teachers if we pay attention,” Dr. Sponaugle said.

“Over that six-year period, she was prescribed multiple antibiotics. Most significant is the fact that she was prescribed four grams of IV Rocephin daily for three years. Unfortunately, the Rocephin was ineffective in killing the massive Bartonella infection.”

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